mouse anti fascin 1  (Santa Cruz Biotechnology)

Journal: Frontiers in Immunology

Article Title: Macrophage subpopulation identity in Drosophila is modulated by apoptotic cell clearance and related signalling pathways

doi: 10.3389/fimmu.2023.1310117

Figure Lengend Snippet: Croquemort and Draper do not affect plasmatocyte subpopulations. (A-D’) representative maximum projection images of the ventral midline of repo -only single mutant embryos (A–D) and crq;repo double mutant embryos (A’-D’) at stage 15. Plasmatocytes labelled via Fascin staining (magenta) while subpopulation plasmatocytes are labelled via VT-GAL4 driving expression of UAS-Stinger (green). Anterior is up in all images, scale bars denote 10µm. (E) scatterplot showing proportion of plasmatocytes within subpopulations. n= 18, 17, 20, 8, 17, 12, 17 and 17, respectively. Statistical analyses carried out via unpaired t -tests. (F-I’) representative maximum projection images of the ventral midline of repo -only single mutant embryos (F–I) and repo mutant embryos expressing UAS-drpr-II specifically in plasmatocytes (F’-I’) at stage 15. All plasmatocytes labelled via srpHemo-H2A-3x-mCherry, while subpopulation plasmatocytes are labelled via VT-GAL4 lines driving expression from UAS-Stinger. Anterior is up in all images, scale bars denote 10µm. (J) scatterplot showing proportion of plasmatocytes within subpopulations. n =14, 19, 17, 13, 17, 14, 16 and 14, respectively. Statistical analyses in (E, J) carried out via unpaired t -tests.

Article Snippet: For Fascin staining, embryos were treated with a mouse anti-Fascin primary antibody (sn7c; Developmental Studies Hybridoma Bank; used at 1:500), with Alexa fluor 568 goat anti-mouse used as a secondary antibody (A11031, Life Technologies; 1:200).

Techniques: Mutagenesis, Staining, Expressing

Journal: Frontiers in Immunology

Article Title: Macrophage subpopulation identity in Drosophila is modulated by apoptotic cell clearance and related signalling pathways

doi: 10.3389/fimmu.2023.1310117

Figure Lengend Snippet: The calcium-permeable cation channel Amo is required for the shift out of the VT57089 subpopulation seen in repo mutants. (A-B”) representative images of the ventral midline of control (A-A”) and amo 1 (B-B”) embryos at stage 15. srpHemo-3x-mCherry labels every plasmatocyte (magenta in merge), while VT57089 subpopulation plasmatocytes labelled via split GAL4 system to drive expression of UAS-eGFP (green in merge). Anterior is up in all images, scale bars denote 10µm. (C) scatterplot showing the proportion of plasmatocytes within the VT57089 subpopulation in control and amo 1 embryos. n =17 and 16, respectively. (D-E”) representative maximum projection images of the ventral midline of repo 03702 single mutant (D-D”) and amo 1 ;repo 03702 double mutant (E-E”) embryos at stage 15. Plasmatocytes have been labelled via Fascin staining (magenta in merge), with VT57089 positive cells labelled via UAS-Stinger (green in merge). Anterior is up in all images, scale bars denote 10µm. (F) scatterplot showing proportion of plasmatocytes within the VT57089 subpopulation between repo 03702 single mutant and amo 1 ;repo 03702 double mutant embryos based on anti-Fascin staining. n =12 and 13, respectively. Statistical analyses in (C, F) carried out via unpaired t -tests. **** represents p<0.0001.

Article Snippet: For Fascin staining, embryos were treated with a mouse anti-Fascin primary antibody (sn7c; Developmental Studies Hybridoma Bank; used at 1:500), with Alexa fluor 568 goat anti-mouse used as a secondary antibody (A11031, Life Technologies; 1:200).

Techniques: Control, Expressing, Mutagenesis, Staining

Journal: Oncology Reports

Article Title: Downregulation of fascin induces collective cell migration in triple‑negative breast cancer

doi: 10.3892/or.2023.8587

Figure Lengend Snippet: Reagents and suppliers.

Article Snippet: Anti-fascin-1 mouse monoclonal antibody , Dako (Agilent Technologies, Inc.) , 1:100.

Techniques: Staining